tmb substrate (Surmodics IVD)
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Structured Review
Surmodics IVD
tmb substrate
Tmb Substrate, supplied by Surmodics IVD, used in various techniques. Bioz Stars score: 95/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+microwell+substrates/TMB+One+Component+HRP+Microwell+Substrate/pmc13106428-247-4-6
Average 95 stars, based on 176 article reviews
Tmb Substrate, supplied by Surmodics IVD, used in various techniques. Bioz Stars score: 95/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+microwell+substrates/TMB+One+Component+HRP+Microwell+Substrate/pmc13106428-247-4-6
Average 95 stars, based on 176 article reviews
tmb substrate - by Bioz Stars,
2026-09
95/100 stars
Images
1) Product Images from "Immunization with detoxified TNFα elicits neutralizing antibodies and ameliorates inflammatory shock and autoimmune arthritis in mice"
Article Title: Immunization with detoxified TNFα elicits neutralizing antibodies and ameliorates inflammatory shock and autoimmune arthritis in mice
Journal: Frontiers in Immunology
doi: 10.3389/fimmu.2026.1801223
Figure Legend Snippet: CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off with PBST. Captured biotinylated mTNFα was detected with streptavidin-HRP and TMB substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Techniques Used: Blocking Assay, Binding Assay, Vaccines, Enzyme-linked Immunosorbent Assay, Inhibition, Incubation
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